Evaluation of the Agronomic Traits of Putative Mv2-Generation Garlic Mutants Produced by Gamma Irradiation in the Highlands
DOI:
https://doi.org/10.54723/ejpgmi.v5i1.697Keywords:
genetic diversity, morphology, putative mutants, acute irradiationAbstract
Garlic has a narrow diversity because it is propagated vegetatively. Mutation breeding can be used to produce diversity in a short time but has the disadvantage of randomness and instability of genetic traits that appear in the next generation. The purpose of this study was to evaluate the agronomic characters and to obtain the MV3 generation garlic putative mutants which have better adaptability to highland areas with better performance. The planting material used was MV2 generation garlic in the form of bulbil and cloves from single technique gamma ray irradiation (Acute Irradition). The garlic genotypes used were Lumbu Kuning, Ciwidey and Tawangmangu Baru. Clove planting material at LD50 and doses above LD50, bulbil LD20. The dosage levels used for cloves were: Tawangmangu Baru 8 Gy (LD50) and wild type (control), Lumbu Kuning 10 Gy and wild type (control), Ciwidey 6 Gy (LD50), 8 Gy (dose above LD50) and wild type ( control). Dosage levels for bulbil: Ciwidey 1.75 Gy and Tawangmangu Baru 0.5 Gy. The results showed that the CWS 8 Gy and LKS 10 Gy mutants had higher number of cloves and tuber weight than the control. Varigata was present only in the CWS 8 Gy mutant. Analysis of the main components of the quantitative and qualitative properties of garlic was reduced to 8 main components.
References
Abdelrahman, M., Hirata, S., & Mukae, T., et all. (2021). COMPREHENSIVE METABOLITE PROFILING IN GENETIC RESOURCES OF GARLIC (ALLIUM SATIVUM L.) COLLECTED FROM DIFFERENT GEOGRAPHICAL REGIONS. Molecules, 26(5), 1415.
Acquah G. (2020). PRINCIPLES OF PLANT GENETICS AND BREEDING. USA. Blackwell Publishing
Ahmadizadeh M,. & Felenji H.,. (2011). EVALUATING YIELD AND SOME TRAITS OF POTATO CULTIVARS IN FALL CULTIVATION OF JIROFT AREA. J. Appl. Environ. Biol. Sci. 1(12). 643-649.
Badan Pusat Statistik (BPS). (2024). Statistik Hortikultura 2024. Jakarta: Badan Pusat Statistik.
Batiha, G. E.-S., Beshbishy, A. M., & Wasef, L. G., et all. (2020). CHEMICAL CONSTITUENTS AND PHARMACOLOGICAL ACTIVITIES OF GARLIC (Allium sativum L.): A review. Nutrients, 12(3), 872. https://doi.org/10.3390/nu12030872
FAO & IAEA. (2023). MANUAL ON MUTATION BREEDING. Vienna: International Atomic Energy Agency.
Kamenetsky-Goldstein, R., & Shemesh-Mayer, E. (2024). STRATEGIES FOR GARLIC (Allium Sativum L.) BREEDING: CHALLENGES AND ACHIEVEMENTS. Acta Horticulturae, 1398, 53–64. https://doi.org/10.17660/ActaHortic.2024.1398.8
Kementerian Pertanian Republik Indonesia. (2023). Statistik Hortikultura Tahun 2023. Jakarta: Direktorat Jenderal Hortikultura.
Kurniajati W. S., Sobir S, & Aisyah S. I,. (2021). PENENTUAN DOSIS IRADIASI SINAR GAMMA DALAM MENINGKATKAN KERAGAMAN UNTUK PERBAIKAN KARAKTER KUANTITATIF BAWANG MERAH (Allium cepa var. aggregatum). J. Ilm. Apl. Isot. dan Radiasi. 16(2):83–90.doi:10.17146/jair.2020.16.2.5962.
Martins, N., et all. (2024). RECENT ADVANCES IN GARLIC RESEARCH: BIOACTIVE COMPOUNDS, BREEDING, AND FUTURE PERSPECTIVES. Scientia Horticulturae.
Norouzi, E., J. Erfani-moghadam, Fazeli A.,Khadivi A,. (2017). MORPHOLOGICAL VARIABILITY WITHIN AND AMONG THREE SPECIES OF ZIZIPHUS GENUS USING MULTIVARIATE ANALYSIS. Sci. Hortic. 222: 180-186.
Oladosu, Y., Rafii, M. Y.,& Abdullah, N., et all. (2021). PRINCIPLE AND APPLICATION OF PLANT MUTAGENESIS IN CROP IMPROVEMENT: A review. Plants, 10, 42.
Pangestuti, P.W., S. Sudarsono, & D. Dinarti,. (2020). DETERMINE THE EFFECT OF GAMMA IRRADIATION TOWARDS THE GROWTH OF TWO LOCAL GARLIC GENOTYPES. IOP Conf. Ser. Earth Environ. Sci. 497(1). Doi:10.1088/1755-1315/497/1/012014
Parry, Martin A. J., Pippa J. Madgwick. P. J., Bayon. C., Tearall. K., Antonio Hernandez., Baudo. M., Rakszegi. M., Hamada. W., Adnan Al-Yassin., Ouabbou. Labhilili. H. M., & Andrew L. Phillips. (2009). MUTATION DISCOVERY FOR CROP IMPROVEMENT. Journal of Experimental Botany. 60(10):2817–25.
Pathirana, R., & Carimi, F. (2022). PLANT MUTATION BREEDING IN THE ERA OF GENOME EDITING. Plant Cell, Tissue and Organ Culture, 149, 451–474
Widyaningtyas N. W., Moeljani I. R., Sulistiyono A., (2021). KERAGAMAN GENETIK TANAMAN BAWANG MERAH (ALLIUM ASCALONICUM L.) VARIETAS BAUJI HASIL IRADIASI SINAR GAMMA 60CO (GENERASI 5).Agrosainstek. 7(2). 48-60.
Zaka R., Chenal C., Misset T. M,. (2004). EFFECTS OF LOW DOSES OF SHORT-TERM GAMMA IRRADIATION ON GROWTH AND DEVELOPMENT THROUGH TWO GENERATIONS OF PISUM SATIVUM. Sci. Total Environ. 320(2). 121–129.











